The relationship between velocity and the substrate concentration is illustrated by an asymptotic curve.
The equation representing this curve is called the Michaelis-Menten equation.
Where V0 = initial velocity, and [S] = concentration of substrate.
In the asymptotic curve, the line approaches but never reaches Vmax. Therefore, it is difficult to calculate. Hence, for more convenience and ease of calculation, the reciprocal of this equation is used to create a straight-line graph called the Lineweaver-Burk Plot.
The Lineweaver Burk Plot can be used to differentiate between the three different types of reversible inhibition, i.e. competitive, uncompetitive and noncompetitive inhibition.
Competitive Inhibition
Uncompetitive Inhibition
Noncompetitive Inhibition
